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Adult Control and Insect Resistance Challenges

Adult Control and Insect Resistance Challenges

98 - Evaluating Insecticide Resistance: comparing topical and bottle bioassays

Wednesday, March 5, 2025
8:50 AM – 9:00 AM AST
Location: 208 B
Abstract: Topical assays, where varying doses of pesticide are placed directly on the cuticle, have been considered the gold standard in determining susceptibility of a population. However, the bottle bioassay, where mosquitoes are placed in a bottle coated with pesticide, is more accessible and more widely used than topical assays. The goal of this study was to determine if there are correlations between the data from bottle bioassays and topical assays, comparing the same active ingredients with the same mosquito species and populations. Preliminary results from three populations show a correlation between LD50 (calculated from the topical assay data) and LT50 (calculated from the bottle bioassay data). Data analysis is ongoing to add more mosquito species, mosquito populations, and pesticide active ingredients.