271 - A Novel Eco friendly Control of Arboviruses Vectors (Aedes aegypti and Ae. albopictus) Using Trachyspermum ammi seed extract -Mediated Silver Nanoparticles (AgNPs)
Thursday, March 6, 2025
10:45 AM – 10:55 AM AST
Location: 208 A
Abstract: Aedes aegypti and Ae. albopictus are the key mosquito species responsible for spreading diseases of public health concern like dengue fever, West Nile virus, Zika virus and chikungunya. As these diseases are viral, so, neither proper vaccine nor treatment is available yet. Therefore, we can avoid these diseases by controlling mosquito population through chemical and biological means. Hence, for this study, acetone extract from Trachyspermum ammi was prepared and used as the reducing agent for the eco-friendly synthesis of silver nanoparticles (AgNPs) by using silver nitrate solution. UV-Vis spectroscopy, Powdered X-ray Diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) were used for the characterization of AgNPs. The 2nd and 3rd instar larvae of Ae. aegypti and Ae. albopictus were exposed to different concentrations (50, 100, 150, 200 & 250 ppm) of plant extract and green synthesized AgNPs to calculate the percentage mortality at time intervals of 6, 12, 18, 24, 30, 36, 42 and 48hr. The Probit analysis of the data proved nanoparticles as an excellent larvicidal agents for both Ae. aegypti and Ae. albopictus with lower LC50 values (76.28 & 80.28 ppm and 87.02 & 89.02 ppm) as compared to the plant extract (121.44 & 127.89 ppm and 127.89 & 129.35 ppm) for 2nd and 3rd instar larvae respectively after 48 hrs exposure time. Our results suggest the extract of T. ammi and synthesized T. ammi AgNPs as excellent controlling agents for vector mosquitoes instead of pollution causing existing chemical pesticides.
