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Poster Session

New Product Trials

Poster Session

P-36 - Oviposition deterrent and ovicidal effects of Salvia munzii essential oil on insecticide-resistant Aedes aegypti (L.)

Wednesday, March 5, 2025
12:15 PM – 1:45 PM AST
Location: Hall A
Abstract: Aedes aegypti, the primary vector of arboviruses such as dengue, Zika, and chikungunya, has developed resistance due to the prolonged use of conventional insecticides. As a result, there is an urgent need to integrate new control tools based on scientific advances in mosquito behavior and chemical ecology. Natural deterrents, such as plant compounds and microbial agents, hold great potential to modify mosquito oviposition behavior, potentially reducing reliance on synthetic insecticides. In this project, we evaluated the oviposition deterrent action of Salvia munzii essential oil on pyrethroid-resistant Ae. aegypti. The study population was selected based on pyrethroid resistance, confirmed through WHO cylinder bioassays. Enzymatic activity was quantified, and kdr mutations conferring resistance at the pyrethroid target site were detected. We assessed the repellent effect (RE), median effective concentration (EC50), ovicidal activity, and oviposition activity index (OAI) in the pyrethroid-resistant population and a susceptible reference strain, New Orleans (NO). The study population showed high resistance to deltamethrin and permethrin, with high frequencies of the mutant alleles I1016 and C1534. At a concentration of 15 ppm, S. munzii oil achieved 100% RE in both the resistant population and the NO strain. The EC50 was 1.63 ppm for the NO strain, while the EC50 for the resistant population was 1.79 ppm. OAI values indicated a strong oviposition deterrence effect in resistant and susceptible strains. Additionally, a 95-100% inhibition of egg hatching was observed in both strains. S. munzii oil proved to be an effective oviposition deterrent and inhibitor of egg hatching in Ae. aegypti.